Journal: Oxidative Medicine and Cellular Longevity

Article Title: Neuroprotective Effects of Palmatine via the Enhancement of Antioxidant Defense and Small Heat Shock Protein Expression in A β -Transgenic Caenorhabditis elegans

doi: 10.1155/2021/9966223

Figure Lengend Snippet: The effects of palmatine on sod-3p ::GFP expression, SOD, and CAT activities. (a, b) The images of sod-3p ::GFP treated with or without palmatine (0.2 mM). (c) Quantification of sod-3p ::GFP fluorescence intensity. The transgenic CF1553 strain was cultured at 20°C for two days on NGM plates with or without palmatine (0.2 mM). The images were obtained by using a Mshot MF52 inverted fluorescence microscope. Quantified sod-3p ::GFP intensity was done by ImageJ software. The result was displayed as mean ± SD ( n = 20–25), and the statistical analysis was carried out with unpaired t -test. ∗∗∗ p < 0.001. (d) The effect of palmatine on the enzyme activity of SOD in wild-type worms. (e) The effect of palmatine on the enzyme activity of CAT in wild-type worms. W ild-type worm s were cultured from L1- to L4-stage at 20°C on NGM plates with or without palmatine (0.2 mM), and then, the worms were lysed to detect the enzyme activities of SOD and CAT. The result was displayed as mean ± SD, and the statistical analysis was done by unpaired t -test. ∗ p < 0.05; ∗∗ p < 0.01.

Article Snippet: The detection assay kits of SOD and CAT enzyme activity and protein quantification (Bicinchoninic acid (BCA)) were acquired from Beyotime (Shanghai, China).

Techniques: Expressing, Fluorescence, Transgenic Assay, Cell Culture, Microscopy, Software, Activity Assay

Journal: Molecules

Article Title: Anti-Aging Effect of Momordica charantia L. on d -Galactose-Induced Subacute Aging in Mice by Activating PI3K/AKT Signaling Pathway

doi: 10.3390/molecules27144502

Figure Lengend Snippet: Effects of MCE on the activity of antioxidant enzymes and the level of lipid peroxidation. SOD ( A ), CAT ( B ), GSH-Px ( C ), MDA ( D ), LPO ( E ). Data were reported as mean ± S.D. of 20 mice in each group. ### p < 0.001, ## p < 0.01, # p < 0.05 vs. control group; ** p < 0.01 vs. d -gal group.

Article Snippet: Commercial kits for the measurement of CAT, SOD, GSH-Px, MDA and protein content (bicinchoninic acid, BCA) were obtained from Nanjing Jiancheng Biotech.

Techniques: Activity Assay, Control

Journal: Experimental Biology and Medicine

Article Title: An experimental model for hypertensive crises emergencies: Long-term high-fat diet followed by acute vasoconstriction stress on spontaneously hypertensive rats

doi: 10.1177/1535370218759270

Figure Lengend Snippet: Myocardial enzymes and energy metabolism tests of the treatment groups at weeks 4, 6, and 8.

Article Snippet: Colorimetric-assay kits for total antioxidant capacity (T-AOC), superoxide dismutase (SOD), methane dicarboxylic aldehyde (MDA), glutathione peroxidase (GSH-PX), myeloperoxidase (MPO), Na + -K + adenosine triphosphatase (ATPase), Ca 2+ ATPase, and bicinchoninic acid protein concentration (BCA) were purchased from Nanjing Jiancheng Bioengineering Institute Co., Ltd, China.

Techniques: Control